Disruption of diatom attachment on marine bioinspired antifouling materials based on Brill (Scophthalmus rhombus).

Bioinspired surfaces, due to their nano and micro topographical features, offer a promising approach for the development of novel antifouling solutions. The study of surface topography has gained popularity in recent years, demonstrating significant potential in mimicking natural structures that could be manufactured for application in the marine environment. This research focuses on investigating the antifouling (AF) performance of bio-inspired micro-textures inspired by Brill fish scales, Scophthalmus rhombus, under static laboratory conditions, using two common fouling diatom species, Amphora coffeaeformis and Nitzschia ovalis. In this study, we evaluate six engineered surfaces, inspired by Brill fish scales, fabricated through a 2-photon polymerization (2PP) process, for their potential as antifouling solutions. The investigation explores the settlement behaviour of microfouling organisms, comparing these mechanisms with theoretical models to guide the future design of antifouling materials. A key emphasis is placed on the impact of surface topography on the disruption of cellular response. Our results suggest that cells smaller than 10 µm, exceeding the peak-to-peak distances between surface features, comfortably position themselves between adjacent features. On the other hand, as peak-to-peak distances decrease, cells shift from settling within uniform gaps to resting on top of surface features. Surfaces with sharpened edges demonstrate a more substantial reduction in diatom attachments compared to those with rounded edges. Furthermore, all micro-textured surfaces exhibit a significant decrease in colony formation compared to control samples. In conclusion, this study shows the potential to manipulate cellular responses through topographical features, providing valuable insights for the design of effective antifouling materials. The results contribute to the growing body of knowledge in biomimetic antifouling strategies using a novel marine organism for inspiration to design practical structures that can be replicated.

A novel low-cost plug-and-play multi-spectral LED based fluorometer, with application to chlorophyll detection.

In this paper a novel low-cost multi-spectral optical fluorometer is presented and evaluated. The device uses a range of LEDs in the blue and violet regions of the electromagnetic spectrum and a mini-spectrometer to detect the emitted fluorescence in the UV to IR spectrum region. Custom built electronics and software were designed to control the system and the components were housed in bespoke 3D printed parts. A number of known fluorophores were tested to determine the capabilities of the fluorometer. Application of the device is demonstrated for the detection of chlorophyll a (Chl a) from laboratory grown algae and from environmental samples while analytical performance is established using both in vivo and extracted Chl a fluorescence and by comparison with a benchtop fluorometer.

Establishment of an antifouling performance index derived from the assessment of biofouling on typical marine sensor materials.

Marine biofouling, known as the unwanted accumulation of living organisms on submerged surfaces, is one of the main factors affecting the operation, maintenance and data quality of water quality monitoring sensors. This can be a significant challenge for marine deployed infrastructure and sensors in water. When organisms attach to the mooring lines or other submerged surfaces of the sensor, they can interfere with the sensor's operation and accuracy. They can also add weight and drag to the mooring system, making it more difficult to maintain the desired position of the sensor. This increases the cost of ownership to the point where it becomes prohibitively expensive to maintain operational sensor networks and infrastructures. Furthermore, the analysis and quantification of biofouling is extremely complex as it is based on biochemical methods such as the analysis of pigments such as chlorophyll-a as a direct indicator of the biomass of photosynthetic organisms, dry weight, carbohydrate analysis and protein analysis among others. In this context, this study has developed a method to estimate biofouling quickly and accurately on different submerged materials used in the marine industry and specifically in sensor manufacturing like copper, titanium, fiberglass composite, different types of polyoxymethylene (POMC, POMH), polyethylene terephthalate glycol (PETG) and 316L-stainless steel. To do this, in situ images of fouling organisms were collected with a conventional camera and image processing algorithms and machine learning models trained were used to construct a biofouling growth model. The algorithms and models were implemented with Fiji-based Weka Segmentation software. A supervised clustering model was used to identify three types of fouling to quantify fouling on panels of different materials submerged in seawater over time. This method is easy, fast and cost-effective to classify biofouling in a more accessible and holistic way that could be useful for engineering applications.

Relativistic Bondi accretion for stiff equations of state.

We revisit Bondi accretion - steady-state, adiabatic, spherical gas flow on to a Schwarzschild black hole at rest in an asymptotically homogeneous medium - for stiff polytropic equations of state (EOSs) with adiabatic indices Γ > 5/3. A general relativistic treatment is required to determine their accretion rates, for which we provide exact expressions. We discuss several qualitative differences between results for soft and stiff EOSs - including the appearance of a minimum steady-state accretion rate for EOSs with Γ ≥ 5/3 - and explore limiting cases in order to examine these differences. As an example, we highlight results for Γ = 2, which is often used in numerical simulations to model the EOS of neutron stars. We also discuss a special case with this index, the ultrarelativistic 'causal' EOS, P = ρ. The latter serves as a useful limit for the still undetermined neutron star EOS above nuclear density. The results are useful, for example, to estimate the accretion rate on to a mini-black hole residing at the centre of a neutron star.

Accretion onto a small black hole at the center of a neutron star.

We revisit the system consisting of a neutron star that harbors a small, possibly primordial, black hole at its center, focusing on a nonspinning black hole embedded in a nonrotating neutron star. Extending earlier treatments, we provide an analytical treatment describing the rate of secular accretion of the neutron star matter onto the black hole, adopting the relativistic Bondi accretion formalism for stiff equations of state that we presented elsewhere. We use these accretion rates to sketch the evolution of the system analytically until the neutron star is completely consumed. We also perform numerical simulations in full general relativity for black holes with masses up to nine orders of magnitude smaller than the neutron star mass, including a simulation of the entire evolution through collapse for the largest black hole mass. We construct relativistic initial data for these simulations by generalizing the black hole puncture method to allow for the presence of matter, and evolve these data with a code that is optimally designed to resolve the vastly different length scales present in this problem. We compare our analytic and numerical results, and provide expressions for the lifetime of neutron stars harboring such endoparasitic black holes.

Targeting the Complement Serine Protease MASP-2 as a Therapeutic Strategy for Coronavirus Infections.

MASP-2, mannose-binding protein-associated serine protease 2, is a key enzyme in the lectin pathway of complement activation. Hyperactivation of this protein by human coronaviruses SARS-CoV, MERS-CoV and SARS-CoV-2 has been found to contribute to aberrant complement activation in patients, leading to aggravated lung injury with potentially fatal consequences. This hyperactivation is triggered in the lungs through a conserved, direct interaction between MASP-2 and coronavirus nucleocapsid (N) proteins. Blocking this interaction with monoclonal antibodies and interfering directly with the catalytic activity of MASP-2, have been found to alleviate coronavirus-induced lung injury both in vitro and in vivo. In this study, a virtual library of 8736 licensed drugs and clinical agents has been screened in silico according to two parallel strategies. The first strategy aims at identifying direct inhibitors of MASP-2 catalytic activity, while the second strategy focusses on finding protein-protein interaction inhibitors (PPIs) of MASP-2 and coronaviral N proteins. Such agents could represent promising support treatment options to prevent lung injury and reduce mortality rates of infections caused by both present and future-emerging coronaviruses. Forty-six drug repurposing candidates were purchased and, for the ones selected as potential direct inhibitors of MASP-2, a preliminary in vitro assay was conducted to assess their interference with the lectin pathway of complement activation. Some of the tested agents displayed a dose-response inhibitory activity of the lectin pathway, potentially providing the basis for a viable support strategy to prevent the severe complications of coronavirus infections.

Bio-inspired Surface Texture Modification as a Viable Feature of Future Aquatic Antifouling Strategies: A Review.

The imitation of natural systems to produce effective antifouling materials is often referred to as "biomimetics". The world of biomimetics is a multidisciplinary one, needing careful understanding of "biological structures", processes and principles of various organisms found in nature and based on this, designing nanodevices and nanomaterials that are of commercial interest to industry. Looking to the marine environment for bioinspired surfaces offers researchers a wealth of topographies to explore. Particular attention has been given to the evaluation of textures based on marine organisms tested in either the laboratory or the field. The findings of the review relate to the numbers of studies on textured surfaces demonstrating antifouling potential which are significant. However, many of these are only tested in the laboratory, where it is acknowledged a very different response to fouling is observed.

Assessment of Antifouling Potential of Novel Transparent Sol Gel Coatings for Application in the Marine Environment.

In recent years, there has become a growing need for the development of antifouling technology for application in the marine environment. The accumulation of large quantities of biomass on these surfaces cause substantial economic burdens within the marine industry, or adversely impact the performance of sensor technologies. Here, we present a study of transparent coatings with potential for applications on sensors or devices with optical windows. The focus of the study is on the abundance and diversity of biofouling organisms that accumulate on glass panels coated with novel transparent or opaque organically modified silicate (ORMOSIL) coatings. The diatom assessment was used to determine the effectiveness of the coatings against biofouling. Test panels were deployed in a marine environment in Galway Bay for durations of nine and thirteen months to examine differences in biofilm formation in both microfouling and macrofouling conditions. The most effective coating is one which consists of precursor, tetraethyl orthosilicate (HC006) that has a water contact angle > 100, without significant roughness (43.52 nm). However, improved roughness and wettability of a second coating, diethoxydimethylsilane (DMDEOS), showed real promise in relation to macrofouling reduction.